General information

Mutant name lec1-2
Mutant/Transgenic plant mutant
Ecotype Ws
Mutagenesis type T-DNA insertion_knock out
Dominant/Recessive/Semi-dominant recessive
PMID 12244265
CommentMutants are desiccation intolerant, have trichomes on cotyledons and exhibit precocious meristem activation.

Mutated genes

Locus name Alias Hormone Mutated site Paper description
AT1G21970 LEC1 abscisic acid T-DNA inserted 115 bp upstream of ORF of LEC1 gene LEAFY COTYLEDON1, transcriptional activator of genes required for both embryo maturation and cellular differentiation.Sequence is similar to HAP3 subunit of the CCAAT-box binding factor.HAP3 subunit is divided into three domains: an amino-terminal A domain, a central B domain, and a carboxyl-terminal C domain. LEC1 shared high similarity with other HAP3 homologs only in central,B domain.LEC1 is required for the specification of cotyledon identity and the completion of embryo maturation.It was sufficient to induce embryogenic programs in vegetative cells, suggesting that LEC1 is a major embryonic regulator that mediates the switch between embryo and vegetative development.

Phenotype information

Organ AttributeNo hormoneabscisic acid
Cotyledon/Leaf otherLeafy cotyledons-
Embryo Defective embryo developmentdefective embryo development-
Silique/Seed Germination rate-slightly ABA-resistant germination
otherseed lethal-