General information

Mutant name lec1-1fus3-3
Mutant/Transgenic plant mutant
Mutagenesis type
PMID 16034595
CommentStrongly impaired ability to induce somatic embryos in vitro.

Mutated genes

Locus name Alias Hormone Mutated site Paper description
AT3G26790 FUS3 abscisic acid A replaced G at the 5`end of the third intron Transcriotional factor with high similarity to the B3 region of the VP1/ABI3-like proteins. Full length FUS3 protein binds to the highly conserved RY motif [DNA motif CATGCA(TG)],present in many seed-specific promoters, and the B3 domains of this transcription factor is necessary for the specific interaction with the RY element.Transcriptional activity of FUS3 requires the B3 DNA-binding domain and an activation domain.
AT1G21970 LEC1 abscisic acid T-DNA inserted 115 bp upstream of ORF of LEC1 gene LEAFY COTYLEDON1, transcriptional activator of genes required for both embryo maturation and cellular differentiation.Sequence is similar to HAP3 subunit of the CCAAT-box binding factor.HAP3 subunit is divided into three domains: an amino-terminal A domain, a central B domain, and a carboxyl-terminal C domain. LEC1 shared high similarity with other HAP3 homologs only in central,B domain.LEC1 is required for the specification of cotyledon identity and the completion of embryo maturation.It was sufficient to induce embryogenic programs in vegetative cells, suggesting that LEC1 is a major embryonic regulator that mediates the switch between embryo and vegetative development.

Phenotype information

Organ AttributeNo hormone
Root otherefficient shoot regenerability via organogenesis from root explants