General information

Mutant name ein3 cul3hyp
Mutant/Transgenic plant mutant
Ecotype Col-0
Mutagenesis type cross of cul3hyp and ein3
Dominant/Recessive/Semi-dominant
PMID 19132805
CommentNo comment

Mutated genes

Locus name Alias Hormone Mutated site Paper description
AT3G20770 EIN3 ethylene EMS nt1598 G to A ETHYLENE-INSENSITIVE3 (EIN3);transcription factor activity;EIN3 acts downstream of the histidine kinase ethylene receptor, ETR1, and the Raf-like kinase, CTR1. The EIN3 gene encodes a novel nuclear-localized protein that shares sequence similarity, structural features, and genetic function with three EIN3-LIKE (EIL) proteins.
AT1G26830 CUL3A ethylene the last two amino acids of the protein are replaced by an eight-residue peptide Cullin, putative, similar to Cullin homolog 3 (CUL-3) SP:Q13618, GI:3639052 from (Homo sapiens); contains Pfam profile PF00888: Cullin family. Interacts with other components of E3 ligase complex suggesting it functions in RUB-modification. Forms complexes with BTB domain proteins forming a novel class of E3-based ubiquitin protein-ligase complexes. Mutant is early flowering and has a reduced sensitivity to far-red light. cul3a/cul3b homozygous/heterozygous plants are embryo lethal.
AT1G69670 CUL3B ethylene T-DNA insertion in the first exon cullin, putative, contains similarity to Cullin homolog 3 (CUL-3) SP:Q13618, GI:3639052 from (Homo sapiens); contains Pfam profile PF00888: Cullin family. Interacts with members of AtBPM family and RBX1 suggesting it is part of an E3 ligase complex involved in RUB modification.

Phenotype information

Organ AttributeNo hormone
Root othernormal, rescued cul3hyp phenotype