General information

Mutant name dsrub
Mutant/Transgenic plant mutant
Ecotype Col-0
Mutagenesis type
Dominant/Recessive/Semi-dominant dominant
PMID 15319484
CommentThe activating enzyme for RUB1 in Arabidopsis is an AXR1/ECR1 heterodimer

Mutated genes

Locus name Alias Hormone Mutated site Paper description
AT1G31340 Related to Ubiquitin 1(RUB1)/Nedd8 auxin dsrub lines were created with a construct containing the RUB1 open reading frame in opposite directions, separated by an intron, under transcriptional control of the 35S promoter of Cauliflower mosaic virus (CaMV35S) ubiquitin-like protein that covalently attaches to cullins

Phenotype information

Organ AttributeNo hormoneauxin
Root Lateral rootreduced-
Primary root-long
otherroots are elongated in dark-grown seedlings-
Hypocotyl/Stem Hypocotyl lengthshort hypocotyls-
Internode lengthshort internode, reduced stem elongation-
Plant heightdwarf, reduced height-
Shoot apical hookenhanced apical hook-
otherWhen grown in the dark dsrub-1, dsrub-2, and dsrub-3 seedlings have shorter, thicker hypocotyls and an exaggerated hook with slightly expanded cotyledons-
Flower Fertilityreduced fertility-